miRNA: 3'- -AAgUCUUGGaCCaCAAGCUGUCa-AUG- -5'
miRNA: 3'- -AAGUCuUGg--ACC-ACAAGCUGUCaAUG- -5'
miRNA: 3'- aagUCUUGGACCACaaGCUGUCaaUg -5'
miRNA: 3'- -AAGUCUUGGACCACAAGCUGUCAAUG- -5'
miRNA: 3'- -AAGUCUUGGACCACAAGCUGUCAAUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3132 | 5' | -48.7 | NC_001493.1 | + | 48182 | 0.66 | 0.99747 |
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Target: 5'- gUUgGGGGCC-GG-GUUCGACAGgcUGCg -3' miRNA: 3'- -AAgUCUUGGaCCaCAAGCUGUCa-AUG- -5' |
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| 3132 | 5' | -48.7 | NC_001493.1 | + | 31630 | 0.67 | 0.995031 |
|
Target: 5'- aUUCGG-ACaggUGGaUGUUCGACAGaUACa -3' miRNA: 3'- -AAGUCuUGg--ACC-ACAAGCUGUCaAUG- -5' |
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| 3132 | 5' | -48.7 | NC_001493.1 | + | 92053 | 0.68 | 0.982601 |
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Target: 5'- --aAGGAUCUGGUGgaCGGCAGggGg -3' miRNA: 3'- aagUCUUGGACCACaaGCUGUCaaUg -5' |
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| 3132 | 5' | -48.7 | NC_001493.1 | + | 9007 | 1.09 | 0.011038 |
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Target: 5'- uUUCAGAACCUGGUGUUCGACAGUUACg -3' miRNA: 3'- -AAGUCUUGGACCACAAGCUGUCAAUG- -5' |
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| 3132 | 5' | -48.7 | NC_001493.1 | + | 124562 | 1.09 | 0.011038 |
|
Target: 5'- uUUCAGAACCUGGUGUUCGACAGUUACg -3' miRNA: 3'- -AAGUCUUGGACCACAAGCUGUCAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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