Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31321 | 5' | -48.7 | NC_006623.1 | + | 38110 | 0.66 | 0.998046 |
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Target: 5'- -aGCCAUG-GAGucggcgacaugcUCCGGCCAGUu -3' miRNA: 3'- ucUGGUACuCUUuuua--------AGGCCGGUCA- -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 22332 | 0.66 | 0.997615 |
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Target: 5'- cAGugCGUGAGAuAAAguugUCUGG-CAGUu -3' miRNA: 3'- -UCugGUACUCUuUUUa---AGGCCgGUCA- -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 46826 | 0.67 | 0.996626 |
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Target: 5'- aGGACgCAaGGGAAAGGUUCgGGuUCAGUu -3' miRNA: 3'- -UCUG-GUaCUCUUUUUAAGgCC-GGUCA- -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 137543 | 0.75 | 0.816111 |
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Target: 5'- cGGACCugcccgGAGGGAGAUUCUGGCgGGc -3' miRNA: 3'- -UCUGGua----CUCUUUUUAAGGCCGgUCa -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 124069 | 0.75 | 0.816111 |
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Target: 5'- cGGACCugcccgGAGGGAGAUUCUGGCgGGc -3' miRNA: 3'- -UCUGGua----CUCUUUUUAAGGCCGgUCa -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 39311 | 0.81 | 0.533354 |
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Target: 5'- aGGGUCGUG-GAGGAGUUCCGGCCGGg -3' miRNA: 3'- -UCUGGUACuCUUUUUAAGGCCGGUCa -5' |
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| 31321 | 5' | -48.7 | NC_006623.1 | + | 66318 | 1.08 | 0.01572 |
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Target: 5'- aAGACCAUGAGAAAAAUUCCGGCCAGUu -3' miRNA: 3'- -UCUGGUACUCUUUUUAAGGCCGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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