Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31322 | 5' | -50.4 | NC_006623.1 | + | 31369 | 0.66 | 0.99604 |
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Target: 5'- uGGUAGGGGcacuuGUugGAUUGGUUGcuCUAUg -3' miRNA: 3'- -CCAUCCUC-----CAugCUAGUCAACc-GAUG- -5' |
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| 31322 | 5' | -50.4 | NC_006623.1 | + | 116557 | 0.67 | 0.98893 |
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Target: 5'- aGUAGGAGGUgggccgcGCGAUCGGgaGuGCc-- -3' miRNA: 3'- cCAUCCUCCA-------UGCUAGUCaaC-CGaug -5' |
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| 31322 | 5' | -50.4 | NC_006623.1 | + | 145055 | 0.67 | 0.98893 |
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Target: 5'- aGUAGGAGGUgggccgcGCGAUCGGgaGuGCc-- -3' miRNA: 3'- cCAUCCUCCA-------UGCUAGUCaaC-CGaug -5' |
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| 31322 | 5' | -50.4 | NC_006623.1 | + | 68188 | 0.67 | 0.987571 |
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Target: 5'- ---cGGGGGUACG-UCuc-UGGCUGCu -3' miRNA: 3'- ccauCCUCCAUGCuAGucaACCGAUG- -5' |
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| 31322 | 5' | -50.4 | NC_006623.1 | + | 81136 | 0.68 | 0.974976 |
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Target: 5'- -aUGGGAuGUGCGA-CGGUUGGCa-- -3' miRNA: 3'- ccAUCCUcCAUGCUaGUCAACCGaug -5' |
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| 31322 | 5' | -50.4 | NC_006623.1 | + | 68322 | 1.12 | 0.005938 |
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Target: 5'- gGGUAGGAGGUACGAUCAGUUGGCUACu -3' miRNA: 3'- -CCAUCCUCCAUGCUAGUCAACCGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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