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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31326 | 3' | -61.2 | NC_006623.1 | + | 68330 | 0.66 | 0.66275 |
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Target: 5'- -uGCCUUGGCgcuCCCCAaaaugagucgggGUGCCUGGGa -3' miRNA: 3'- uuCGGGACCG---GGGGUga----------UAUGGGCCU- -5' |
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| 31326 | 3' | -61.2 | NC_006623.1 | + | 64119 | 0.66 | 0.626622 |
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Target: 5'- cAGGCUCcGcGCCCCCaACUucaaugGCCUGGAc -3' miRNA: 3'- -UUCGGGaC-CGGGGG-UGAua----UGGGCCU- -5' |
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| 31326 | 3' | -61.2 | NC_006623.1 | + | 124134 | 0.67 | 0.566681 |
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Target: 5'- aAGGCCCUcgccgcGGCCCaggCCACUAU-UCCGGc -3' miRNA: 3'- -UUCGGGA------CCGGG---GGUGAUAuGGGCCu -5' |
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| 31326 | 3' | -61.2 | NC_006623.1 | + | 137478 | 0.67 | 0.566681 |
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Target: 5'- aAGGCCCUcgccgcGGCCCaggCCACUAU-UCCGGc -3' miRNA: 3'- -UUCGGGA------CCGGG---GGUGAUAuGGGCCu -5' |
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| 31326 | 3' | -61.2 | NC_006623.1 | + | 53856 | 0.67 | 0.556809 |
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Target: 5'- cGGGCCa-GGCUgUCAUUGUugCCGGAu -3' miRNA: 3'- -UUCGGgaCCGGgGGUGAUAugGGCCU- -5' |
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| 31326 | 3' | -61.2 | NC_006623.1 | + | 113084 | 1.06 | 0.001258 |
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Target: 5'- aAAGCCCUGGCCCCCACUAUACCCGGAa -3' miRNA: 3'- -UUCGGGACCGGGGGUGAUAUGGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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