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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31328 | 3' | -51.8 | NC_006623.1 | + | 146682 | 0.78 | 0.534802 |
|
Target: 5'- -uCUUCUCGUCccCGUCUUCUUCAGAu -3' miRNA: 3'- acGAGGAGCAGcaGCAGAAGAAGUCU- -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 146473 | 1.1 | 0.006854 |
|
Target: 5'- aUGCUCCUCGUCGUCGUCUUCUUCAGAc -3' miRNA: 3'- -ACGAGGAGCAGCAGCAGAAGAAGUCU- -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 134869 | 0.67 | 0.979703 |
|
Target: 5'- cGUUCUUCGUCGUCuugcgagaccguGUCUaaUUUUCGGu -3' miRNA: 3'- aCGAGGAGCAGCAG------------CAGA--AGAAGUCu -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 133646 | 0.67 | 0.977349 |
|
Target: 5'- gUGCucggugUCCUCGgggacuUCGUCGUCUUCgg-GGAg -3' miRNA: 3'- -ACG------AGGAGC------AGCAGCAGAAGaagUCU- -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 115139 | 1.1 | 0.006854 |
|
Target: 5'- aUGCUCCUCGUCGUCGUCUUCUUCAGAc -3' miRNA: 3'- -ACGAGGAGCAGCAGCAGAAGAAGUCU- -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 114930 | 0.78 | 0.534802 |
|
Target: 5'- -uCUUCUCGUCccCGUCUUCUUCAGAu -3' miRNA: 3'- acGAGGAGCAGcaGCAGAAGAAGUCU- -5' |
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| 31328 | 3' | -51.8 | NC_006623.1 | + | 9003 | 0.72 | 0.851704 |
|
Target: 5'- gUGCUCCUCGgUGUUGUcCUUCcaCGGAa -3' miRNA: 3'- -ACGAGGAGCaGCAGCA-GAAGaaGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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