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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31329 | 5' | -53.1 | NC_006623.1 | + | 138548 | 0.66 | 0.962506 |
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Target: 5'- --aGugGGUGAGGG---GGGAGAguGg -3' miRNA: 3'- uugCugCUACUCCCaugUCCUCUguC- -5' |
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| 31329 | 5' | -53.1 | NC_006623.1 | + | 123064 | 0.66 | 0.962506 |
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Target: 5'- --aGugGGUGAGGG---GGGAGAguGg -3' miRNA: 3'- uugCugCUACUCCCaugUCCUCUguC- -5' |
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| 31329 | 5' | -53.1 | NC_006623.1 | + | 123141 | 0.69 | 0.874978 |
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Target: 5'- uGGCGGCGA-GAGGGUguuGCGGGGGuaaAUGGg -3' miRNA: 3'- -UUGCUGCUaCUCCCA---UGUCCUC---UGUC- -5' |
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| 31329 | 5' | -53.1 | NC_006623.1 | + | 138471 | 0.69 | 0.874978 |
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Target: 5'- uGGCGGCGA-GAGGGUguuGCGGGGGuaaAUGGg -3' miRNA: 3'- -UUGCUGCUaCUCCCA---UGUCCUC---UGUC- -5' |
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| 31329 | 5' | -53.1 | NC_006623.1 | + | 146209 | 1.07 | 0.006662 |
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Target: 5'- cAACGACGAUGAGGGUACAGGAGACAGa -3' miRNA: 3'- -UUGCUGCUACUCCCAUGUCCUCUGUC- -5' |
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| 31329 | 5' | -53.1 | NC_006623.1 | + | 115403 | 1.07 | 0.006662 |
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Target: 5'- cAACGACGAUGAGGGUACAGGAGACAGa -3' miRNA: 3'- -UUGCUGCUACUCCCAUGUCCUCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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