Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31334 | 5' | -55.6 | NC_006623.1 | + | 115423 | 0.7 | 0.776361 |
Target: 5'- cCGCC-CGGUAgguAUGCGCGGACccgaGCGGGa -3' miRNA: 3'- -GUGGcGUUAU---UGCGUGCCUG----CGCCCc -5' |
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31334 | 5' | -55.6 | NC_006623.1 | + | 124192 | 0.72 | 0.658881 |
Target: 5'- uCGCCGCuaacgucGGCGcCGCGGGgaGCGGGGu -3' miRNA: 3'- -GUGGCGuua----UUGC-GUGCCUg-CGCCCC- -5' |
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31334 | 5' | -55.6 | NC_006623.1 | + | 311 | 0.72 | 0.658881 |
Target: 5'- gCugCGCAcgAACGCAgGGccGCGCuggcaGGGGg -3' miRNA: 3'- -GugGCGUuaUUGCGUgCC--UGCG-----CCCC- -5' |
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31334 | 5' | -55.6 | NC_006623.1 | + | 139920 | 1.11 | 0.002694 |
Target: 5'- gCACCGCAAUAACGCACGGACGCGGGGg -3' miRNA: 3'- -GUGGCGUUAUUGCGUGCCUGCGCCCC- -5' |
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31334 | 5' | -55.6 | NC_006623.1 | + | 121692 | 1.11 | 0.002694 |
Target: 5'- gCACCGCAAUAACGCACGGACGCGGGGg -3' miRNA: 3'- -GUGGCGUUAUUGCGUGCCUGCGCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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