miRNA: 3'- aUACCUaaUCUCGGGUCGu-GC---AUCUG- -5'
miRNA: 3'- auACCUaaUCUCGGGUCGUGC-AUCUg -5'
miRNA: 3'- -AUACCUAAUCUCGGGUCGUGCAUCUG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31338 | 3' | -51.5 | NC_006623.1 | + | 26165 | 0.66 | 0.978868 |
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Target: 5'- gGUGGAaaAGGGCCUGGCuuCGagaUAGGCg -3' miRNA: 3'- aUACCUaaUCUCGGGUCGu-GC---AUCUG- -5' |
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| 31338 | 3' | -51.5 | NC_006623.1 | + | 133464 | 0.67 | 0.964254 |
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Target: 5'- -cUGGAcgAGGGUCUGGCGCGcUAGGa -3' miRNA: 3'- auACCUaaUCUCGGGUCGUGC-AUCUg -5' |
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| 31338 | 3' | -51.5 | NC_006623.1 | + | 128998 | 1.09 | 0.005752 |
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Target: 5'- uUAUGGAUUAGAGCCCAGCACGUAGACa -3' miRNA: 3'- -AUACCUAAUCUCGGGUCGUGCAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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