miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31339 3' -56 NC_006623.1 + 136891 0.66 0.929209
Target:  5'- cCCGCGcGCGCUGCagaauuuaGGGUUC-CagGGa -3'
miRNA:   3'- -GGCGCuCGUGGCGg-------UCCAAGaGaaCC- -5'
31339 3' -56 NC_006623.1 + 3880 0.66 0.923934
Target:  5'- aCUGCG-GCACUGCUAGGggcgccgCUCa--- -3'
miRNA:   3'- -GGCGCuCGUGGCGGUCCaa-----GAGaacc -5'
31339 3' -56 NC_006623.1 + 17036 0.7 0.726048
Target:  5'- -gGgGAGCGCCGCaGGGUUCacaauauccagCUUGGg -3'
miRNA:   3'- ggCgCUCGUGGCGgUCCAAGa----------GAACC- -5'
31339 3' -56 NC_006623.1 + 129890 1.12 0.001956
Target:  5'- uCCGCGAGCACCGCCAGGUUCUCUUGGa -3'
miRNA:   3'- -GGCGCUCGUGGCGGUCCAAGAGAACC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.