Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31344 | 5' | -48.3 | NC_006623.1 | + | 76016 | 0.66 | 0.998722 |
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Target: 5'- uAGCUcUCCAUGGa-UCUaGCgAAGggGGGa -3' miRNA: 3'- -UCGA-AGGUACCgaAGA-UG-UUCuuCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 113293 | 0.67 | 0.99774 |
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Target: 5'- cGCUUCCuuauaagGGUUcCUGCGacgguAGAAGAGu -3' miRNA: 3'- uCGAAGGua-----CCGAaGAUGU-----UCUUCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 148319 | 0.67 | 0.99774 |
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Target: 5'- cGCUUCCuuauaagGGUUcCUGCGacgguAGAAGAGu -3' miRNA: 3'- uCGAAGGua-----CCGAaGAUGU-----UCUUCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 146671 | 0.68 | 0.992874 |
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Target: 5'- cGCUUCCuuuagGGCcgucgCUGCGuGGggGAGg -3' miRNA: 3'- uCGAAGGua---CCGaa---GAUGU-UCuuCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 114941 | 0.68 | 0.992874 |
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Target: 5'- cGCUUCCuuuagGGCcgucgCUGCGuGGggGAGg -3' miRNA: 3'- uCGAAGGua---CCGaa---GAUGU-UCuuCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 52927 | 0.68 | 0.991759 |
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Target: 5'- aAGUcagCCAUGGUUUCUACGA-AAGAa -3' miRNA: 3'- -UCGaa-GGUACCGAAGAUGUUcUUCUc -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 15305 | 0.71 | 0.957582 |
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Target: 5'- uGGCaa-CAUGGCcugaUCUGCGAGggGGGc -3' miRNA: 3'- -UCGaagGUACCGa---AGAUGUUCuuCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 116809 | 1.09 | 0.012582 |
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Target: 5'- cAGCUUCCAUGGCUUCUACAAGAAGAGu -3' miRNA: 3'- -UCGAAGGUACCGAAGAUGUUCUUCUC- -5' |
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| 31344 | 5' | -48.3 | NC_006623.1 | + | 144802 | 1.09 | 0.012582 |
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Target: 5'- cAGCUUCCAUGGCUUCUACAAGAAGAGu -3' miRNA: 3'- -UCGAAGGUACCGAAGAUGUUCUUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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