Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31345 | 5' | -61.7 | NC_006623.1 | + | 1693 | 0.66 | 0.659881 |
Target: 5'- gUCCUGCCGucCUGCaccuuUGCUgu-CCCGCa -3' miRNA: 3'- -AGGGCGGCcuGGCG-----ACGAguuGGGCG- -5' |
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31345 | 5' | -61.7 | NC_006623.1 | + | 66728 | 0.66 | 0.667702 |
Target: 5'- gCCgCGCCGG-CCGCaGCUacaaaacucaaaGACaCCGCu -3' miRNA: 3'- aGG-GCGGCCuGGCGaCGAg-----------UUG-GGCG- -5' |
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31345 | 5' | -61.7 | NC_006623.1 | + | 140094 | 0.66 | 0.673557 |
Target: 5'- cCCCGCguccgugcguuauugCGGugCGCgGC---ACCCGCa -3' miRNA: 3'- aGGGCG---------------GCCugGCGaCGaguUGGGCG- -5' |
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31345 | 5' | -61.7 | NC_006623.1 | + | 145935 | 0.66 | 0.6794 |
Target: 5'- gUCCGCagaGGGCCGCguagGCgu-ACgCGCg -3' miRNA: 3'- aGGGCGg--CCUGGCGa---CGaguUGgGCG- -5' |
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31345 | 5' | -61.7 | NC_006623.1 | + | 145693 | 1.11 | 0.000615 |
Target: 5'- uUCCCGCCGGACCGCUGCUCAACCCGCg -3' miRNA: 3'- -AGGGCGGCCUGGCGACGAGUUGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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