Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31359 | 3' | -54.6 | NC_006623.1 | + | 33104 | 0.67 | 0.906902 |
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Target: 5'- aUUUUCGUGGuuGGAugGuaGCCUCGCu -3' miRNA: 3'- -AAGAGCAUUgcCCUugCucCGGAGCG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 38429 | 0.67 | 0.906902 |
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Target: 5'- cUCUCGUGAUGGcGAcACG-GGUCUC-Cg -3' miRNA: 3'- aAGAGCAUUGCC-CU-UGCuCCGGAGcG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 79650 | 0.68 | 0.887197 |
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Target: 5'- --aUCGUGucuuuacCGGGggUGAGGCUUCa- -3' miRNA: 3'- aagAGCAUu------GCCCuuGCUCCGGAGcg -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 130309 | 0.68 | 0.880164 |
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Target: 5'- -aCUCGUcGCGGGAgaACGGGcaGCC-CGUg -3' miRNA: 3'- aaGAGCAuUGCCCU--UGCUC--CGGaGCG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 134815 | 0.68 | 0.865427 |
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Target: 5'- uUUCUgGUGACGauGGAaauuacaaugACGAGGCCgaucguugCGCu -3' miRNA: 3'- -AAGAgCAUUGC--CCU----------UGCUCCGGa-------GCG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 131354 | 0.69 | 0.849834 |
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Target: 5'- -aCUCGgacucagagcGCGGGAACuGAGGCCUUu- -3' miRNA: 3'- aaGAGCau--------UGCCCUUG-CUCCGGAGcg -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 67666 | 0.69 | 0.833435 |
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Target: 5'- aUUUUGacauaaggGAUGGGcGCGAgGGCCUCGCc -3' miRNA: 3'- aAGAGCa-------UUGCCCuUGCU-CCGGAGCG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 136071 | 0.69 | 0.816288 |
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Target: 5'- cUUCUagaGUAucGCGGGAACaauccagcaaaGAGGCCgCGCc -3' miRNA: 3'- -AAGAg--CAU--UGCCCUUG-----------CUCCGGaGCG- -5' |
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| 31359 | 3' | -54.6 | NC_006623.1 | + | 12005 | 1.08 | 0.004128 |
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Target: 5'- gUUCUCGUAACGGGAACGAGGCCUCGCc -3' miRNA: 3'- -AAGAGCAUUGCCCUUGCUCCGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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