Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31361 | 5' | -59.8 | NC_006623.1 | + | 139775 | 0.66 | 0.753211 |
Target: 5'- aGAaGCGGacgcgcgccccuuuGcgACCGCCGgacauCCCGCCGCCc -3' miRNA: 3'- -CUcCGCU--------------CuaUGGUGGU-----GGGCGGCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 145022 | 0.7 | 0.540419 |
Target: 5'- gGAGGUaGGucGUACCGgcaagucguCCGCCgGCCGCCu -3' miRNA: 3'- -CUCCGcUC--UAUGGU---------GGUGGgCGGCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 145117 | 0.67 | 0.702439 |
Target: 5'- -uGGCGGGAgauucuuggGCgGCCGaaggcucugcuccguCgCCGCCGCCa -3' miRNA: 3'- cuCCGCUCUa--------UGgUGGU---------------G-GGCGGCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 148417 | 0.66 | 0.756012 |
Target: 5'- cAGGCauuc--CCACCGCgcgcaaCCGCCGCCg -3' miRNA: 3'- cUCCGcucuauGGUGGUG------GGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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