Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31361 | 5' | -59.8 | NC_006623.1 | + | 139370 | 0.71 | 0.465276 |
Target: 5'- cGAGGCGAcagcGGcACCuuGCCGCCUGCgcuCGCCa -3' miRNA: 3'- -CUCCGCU----CUaUGG--UGGUGGGCG---GCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 122242 | 0.71 | 0.465276 |
Target: 5'- cGAGGCGAcagcGGcACCuuGCCGCCUGCgcuCGCCa -3' miRNA: 3'- -CUCCGCU----CUaUGG--UGGUGGGCG---GCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 110531 | 0.74 | 0.319196 |
Target: 5'- aGGGCGGGAggGCCGagaGCCCGCaGCCg -3' miRNA: 3'- cUCCGCUCUa-UGGUgg-UGGGCGgCGG- -5' |
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31361 | 5' | -59.8 | NC_006623.1 | + | 19788 | 1.1 | 0.001169 |
Target: 5'- gGAGGCGAGAUACCACCACCCGCCGCCc -3' miRNA: 3'- -CUCCGCUCUAUGGUGGUGGGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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