Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31362 | 3' | -57.2 | NC_006623.1 | + | 125890 | 0.66 | 0.857153 |
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Target: 5'- -aGCgGGAGCCCUUCGAauaUGGAuGGGUg -3' miRNA: 3'- ggCGgCUUCGGGGGGCU---ACCU-UUCAa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 118867 | 0.66 | 0.849366 |
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Target: 5'- aCG-UGAAGCUUUCUGGUGGGGAGUa -3' miRNA: 3'- gGCgGCUUCGGGGGGCUACCUUUCAa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 142744 | 0.66 | 0.849366 |
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Target: 5'- aCG-UGAAGCUUUCUGGUGGGGAGUa -3' miRNA: 3'- gGCgGCUUCGGGGGGCUACCUUUCAa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 128814 | 0.67 | 0.824866 |
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Target: 5'- aCGCCuGGGCuauaauuuugCCCCCGAacgGGAAAGg- -3' miRNA: 3'- gGCGGcUUCG----------GGGGGCUa--CCUUUCaa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 139883 | 0.68 | 0.732239 |
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Target: 5'- gCgGCCGAAGCCUUCCG-UGGGucuuugcGGGUg -3' miRNA: 3'- -GgCGGCUUCGGGGGGCuACCU-------UUCAa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 121729 | 0.68 | 0.732239 |
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Target: 5'- gCgGCCGAAGCCUUCCG-UGGGucuuugcGGGUg -3' miRNA: 3'- -GgCGGCUUCGGGGGGCuACCU-------UUCAa -5' |
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| 31362 | 3' | -57.2 | NC_006623.1 | + | 19967 | 1.06 | 0.003268 |
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Target: 5'- gCCGCCGAAGCCCCCCGAUGGAAAGUUu -3' miRNA: 3'- -GGCGGCUUCGGGGGGCUACCUUUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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