Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31363 | 3' | -56.8 | NC_006623.1 | + | 141849 | 0.66 | 0.903622 |
Target: 5'- aGGCaCACaCCCCGGCaCCGAauuGCAc-- -3' miRNA: 3'- cCUG-GUG-GGGGCCG-GGCUau-UGUacu -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 39259 | 0.68 | 0.805464 |
Target: 5'- uGACUACCCCCuGCUCGAauacccuuCGUGGg -3' miRNA: 3'- cCUGGUGGGGGcCGGGCUauu-----GUACU- -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 9979 | 0.69 | 0.750539 |
Target: 5'- --uCCcUCCCCGGCCCucuGUAAUAUGAg -3' miRNA: 3'- ccuGGuGGGGGCCGGGc--UAUUGUACU- -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 87983 | 0.69 | 0.718654 |
Target: 5'- aGACCAUaCCUGGCCCcuguucauguggagGAUGACGUGu -3' miRNA: 3'- cCUGGUGgGGGCCGGG--------------CUAUUGUACu -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 111670 | 0.73 | 0.511466 |
Target: 5'- cGGGCCGCUgCCauuucaacgaacgGGCCCGAUGGCgcuGUGGg -3' miRNA: 3'- -CCUGGUGGgGG-------------CCGGGCUAUUG---UACU- -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 16726 | 0.76 | 0.386794 |
Target: 5'- aGACCugCCCUggagGGCCUGAUGcuucaGCAUGAg -3' miRNA: 3'- cCUGGugGGGG----CCGGGCUAU-----UGUACU- -5' |
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31363 | 3' | -56.8 | NC_006623.1 | + | 24014 | 1.11 | 0.001854 |
Target: 5'- cGGACCACCCCCGGCCCGAUAACAUGAa -3' miRNA: 3'- -CCUGGUGGGGGCCGGGCUAUUGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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