Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31364 | 3' | -56 | NC_006623.1 | + | 44207 | 0.66 | 0.881146 |
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Target: 5'- uAGCUCaCugAGCAUaCUuuGUGCGGUa -3' miRNA: 3'- -UCGAGcGugUCGUAaGGggUACGCUA- -5' |
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| 31364 | 3' | -56 | NC_006623.1 | + | 30022 | 0.66 | 0.876783 |
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Target: 5'- uGCUUGCACAGCAgugUCCgaagcauggaagaaCAUGgGAa -3' miRNA: 3'- uCGAGCGUGUCGUa--AGGg-------------GUACgCUa -5' |
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| 31364 | 3' | -56 | NC_006623.1 | + | 75489 | 0.66 | 0.842355 |
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Target: 5'- -uCUCGCGCuaggAGUGUUUCCaCGUGCGAa -3' miRNA: 3'- ucGAGCGUG----UCGUAAGGG-GUACGCUa -5' |
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| 31364 | 3' | -56 | NC_006623.1 | + | 79625 | 0.67 | 0.816631 |
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Target: 5'- --aUCGCGCAGCAUUCCCaagaucUGCu-- -3' miRNA: 3'- ucgAGCGUGUCGUAAGGGgu----ACGcua -5' |
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| 31364 | 3' | -56 | NC_006623.1 | + | 10937 | 0.69 | 0.700487 |
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Target: 5'- gAGCUgCGCACAaaccacucuGCAUUCCUCuuUGCGGc -3' miRNA: 3'- -UCGA-GCGUGU---------CGUAAGGGGu-ACGCUa -5' |
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| 31364 | 3' | -56 | NC_006623.1 | + | 101431 | 1.05 | 0.003949 |
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Target: 5'- cAGCUCGCACAGCAUUCCCCAUGCGAUc -3' miRNA: 3'- -UCGAGCGUGUCGUAAGGGGUACGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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