Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31365 | 5' | -54.5 | NC_006623.1 | + | 93197 | 0.66 | 0.92636 |
Target: 5'- aUGUUGcuGCCCCUGCuguucaaaguccuGCGGGggugACACAa -3' miRNA: 3'- aAUAAU--CGGGGGCG-------------CGCCUa---UGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 116000 | 0.66 | 0.921276 |
Target: 5'- ---aUGGCUCCCcUGCGGAgaGCGCAa -3' miRNA: 3'- aauaAUCGGGGGcGCGCCUa-UGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 145612 | 0.66 | 0.921276 |
Target: 5'- ---aUGGCUCCCcUGCGGAgaGCGCAa -3' miRNA: 3'- aauaAUCGGGGGcGCGCCUa-UGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 124 | 0.72 | 0.625568 |
Target: 5'- --uUUGGCCCCCugccaGCGCGGcccugcguucGUGCGCAg -3' miRNA: 3'- aauAAUCGGGGG-----CGCGCC----------UAUGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 51368 | 0.73 | 0.551991 |
Target: 5'- ---aUGGCCCCCGUaauaccuucucGuCGGAUGCGCAg -3' miRNA: 3'- aauaAUCGGGGGCG-----------C-GCCUAUGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 11083 | 0.75 | 0.442821 |
Target: 5'- ---aUGGCCUCUGauCGCGGAUACACAa -3' miRNA: 3'- aauaAUCGGGGGC--GCGCCUAUGUGUa -5' |
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31365 | 5' | -54.5 | NC_006623.1 | + | 109903 | 1.04 | 0.006514 |
Target: 5'- cUUAUUAGCCCCCGCGCGGAUACACAUg -3' miRNA: 3'- -AAUAAUCGGGGGCGCGCCUAUGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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