miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31365 5' -54.5 NC_006623.1 + 93197 0.66 0.92636
Target:  5'- aUGUUGcuGCCCCUGCuguucaaaguccuGCGGGggugACACAa -3'
miRNA:   3'- aAUAAU--CGGGGGCG-------------CGCCUa---UGUGUa -5'
31365 5' -54.5 NC_006623.1 + 116000 0.66 0.921276
Target:  5'- ---aUGGCUCCCcUGCGGAgaGCGCAa -3'
miRNA:   3'- aauaAUCGGGGGcGCGCCUa-UGUGUa -5'
31365 5' -54.5 NC_006623.1 + 145612 0.66 0.921276
Target:  5'- ---aUGGCUCCCcUGCGGAgaGCGCAa -3'
miRNA:   3'- aauaAUCGGGGGcGCGCCUa-UGUGUa -5'
31365 5' -54.5 NC_006623.1 + 124 0.72 0.625568
Target:  5'- --uUUGGCCCCCugccaGCGCGGcccugcguucGUGCGCAg -3'
miRNA:   3'- aauAAUCGGGGG-----CGCGCC----------UAUGUGUa -5'
31365 5' -54.5 NC_006623.1 + 51368 0.73 0.551991
Target:  5'- ---aUGGCCCCCGUaauaccuucucGuCGGAUGCGCAg -3'
miRNA:   3'- aauaAUCGGGGGCG-----------C-GCCUAUGUGUa -5'
31365 5' -54.5 NC_006623.1 + 11083 0.75 0.442821
Target:  5'- ---aUGGCCUCUGauCGCGGAUACACAa -3'
miRNA:   3'- aauaAUCGGGGGC--GCGCCUAUGUGUa -5'
31365 5' -54.5 NC_006623.1 + 109903 1.04 0.006514
Target:  5'- cUUAUUAGCCCCCGCGCGGAUACACAUg -3'
miRNA:   3'- -AAUAAUCGGGGGCGCGCCUAUGUGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.