Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31367 | 5' | -54.3 | NC_006623.1 | + | 50 | 0.66 | 0.910434 |
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Target: 5'- -cUCaauagaAGCgAACGggCGCUGGCGAGAu -3' miRNA: 3'- aaAGg-----UCG-UUGCgaGCGACCGUUCUu -5' |
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| 31367 | 5' | -54.3 | NC_006623.1 | + | 1049 | 0.66 | 0.910434 |
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Target: 5'- -cUCaauagaAGCgAACGggCGCUGGCGAGAu -3' miRNA: 3'- aaAGg-----UCG-UUGCgaGCGACCGUUCUu -5' |
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| 31367 | 5' | -54.3 | NC_006623.1 | + | 112591 | 0.67 | 0.868053 |
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Target: 5'- aUUCCAGUguGGCGCUUaUUGuGCAAGAAc -3' miRNA: 3'- aAAGGUCG--UUGCGAGcGAC-CGUUCUU- -5' |
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| 31367 | 5' | -54.3 | NC_006623.1 | + | 131399 | 0.7 | 0.72952 |
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Target: 5'- -cUCCGGCGccGCGCUCGgaGcGCGAGc- -3' miRNA: 3'- aaAGGUCGU--UGCGAGCgaC-CGUUCuu -5' |
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| 31367 | 5' | -54.3 | NC_006623.1 | + | 82675 | 0.75 | 0.438824 |
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Target: 5'- -gUCgCGGCAuacaGCUUGCUGGCAGGAu -3' miRNA: 3'- aaAG-GUCGUug--CGAGCGACCGUUCUu -5' |
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| 31367 | 5' | -54.3 | NC_006623.1 | + | 112449 | 1.04 | 0.006081 |
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Target: 5'- aUUUCCAGCAACGCUCGCUGGCAAGAAc -3' miRNA: 3'- -AAAGGUCGUUGCGAGCGACCGUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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