Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31368 | 3' | -49.6 | NC_006623.1 | + | 7308 | 0.66 | 0.995214 |
Target: 5'- uGCCGCccaggAGGUauGCUACagGGGUUu -3' miRNA: 3'- -CGGCGuuaa-UCCA--CGAUGagUCCAAu -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 112364 | 0.66 | 0.994407 |
Target: 5'- cGCgGCAGcgcgAGGUGCUuuGCUCAGu--- -3' miRNA: 3'- -CGgCGUUaa--UCCACGA--UGAGUCcaau -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 123453 | 0.67 | 0.986315 |
Target: 5'- cGCCGCGAguccggcggccGGcgcGCUGCUCuGGUUAu -3' miRNA: 3'- -CGGCGUUaau--------CCa--CGAUGAGuCCAAU- -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 138159 | 0.67 | 0.986315 |
Target: 5'- cGCCGCGAguccggcggccGGcgcGCUGCUCuGGUUAu -3' miRNA: 3'- -CGGCGUUaau--------CCa--CGAUGAGuCCAAU- -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 123142 | 0.68 | 0.978887 |
Target: 5'- gGCgGCGAgaGGGUGUUGCggGGGUa- -3' miRNA: 3'- -CGgCGUUaaUCCACGAUGagUCCAau -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 138470 | 0.68 | 0.978887 |
Target: 5'- gGCgGCGAgaGGGUGUUGCggGGGUa- -3' miRNA: 3'- -CGgCGUUaaUCCACGAUGagUCCAau -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 132442 | 0.71 | 0.927629 |
Target: 5'- aUUGCGGUggaaaacGGUGCUGCUCAGGc-- -3' miRNA: 3'- cGGCGUUAau-----CCACGAUGAGUCCaau -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 329 | 0.74 | 0.805164 |
Target: 5'- uGCCGCGGggcaggGGGUGCUGCgcacgaacgCAGGg-- -3' miRNA: 3'- -CGGCGUUaa----UCCACGAUGa--------GUCCaau -5' |
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31368 | 3' | -49.6 | NC_006623.1 | + | 112676 | 1.1 | 0.008654 |
Target: 5'- gGCCGCAAUUAGGUGCUACUCAGGUUAa -3' miRNA: 3'- -CGGCGUUAAUCCACGAUGAGUCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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