miRNA: 3'- -GAGACAg-AGGACa---UGGGAGUa---GCAGc -5'
miRNA: 3'- gaGACAGAGgACauGGGAGUaGCAGC- -5'
miRNA: 3'- gaGACAGAGgACauGGGAGUaGCAGC- -5'
miRNA: 3'- -GAGACAGAGGACAUGGGAGUAGCAGC- -5'
miRNA: 3'- -GAGACAGAGGACAUGGGAGUAGCAGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31371 | 3' | -53 | NC_006623.1 | + | 108461 | 0.67 | 0.956498 |
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Target: 5'- gCUCUGUagUCCUGaaggACCUUCAccugCGUCa -3' miRNA: 3'- -GAGACAg-AGGACa---UGGGAGUa---GCAGc -5' |
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| 31371 | 3' | -53 | NC_006623.1 | + | 146174 | 0.7 | 0.878873 |
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Target: 5'- -aCUGUCUCaUGgcCCCUCAgCGUUGg -3' miRNA: 3'- gaGACAGAGgACauGGGAGUaGCAGC- -5' |
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| 31371 | 3' | -53 | NC_006623.1 | + | 115438 | 0.7 | 0.878873 |
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Target: 5'- -aCUGUCUCaUGgcCCCUCAgCGUUGg -3' miRNA: 3'- gaGACAGAGgACauGGGAGUaGCAGC- -5' |
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| 31371 | 3' | -53 | NC_006623.1 | + | 115226 | 1.09 | 0.006249 |
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Target: 5'- uCUCUGUCUCCUGUACCCUCAUCGUCGu -3' miRNA: 3'- -GAGACAGAGGACAUGGGAGUAGCAGC- -5' |
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| 31371 | 3' | -53 | NC_006623.1 | + | 146386 | 1.09 | 0.006249 |
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Target: 5'- uCUCUGUCUCCUGUACCCUCAUCGUCGu -3' miRNA: 3'- -GAGACAGAGGACAUGGGAGUAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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