Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31371 | 5' | -56.5 | NC_006623.1 | + | 146208 | 0.68 | 0.79249 |
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Target: 5'- -aCAACGacgaUGAGGGuaCAgGAGACAGa -3' miRNA: 3'- ggGUUGCg---ACUCCCcgGUaCUCUGUC- -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 115404 | 0.68 | 0.79249 |
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Target: 5'- -aCAACGacgaUGAGGGuaCAgGAGACAGa -3' miRNA: 3'- ggGUUGCg---ACUCCCcgGUaCUCUGUC- -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 108622 | 0.68 | 0.787035 |
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Target: 5'- gCCCuGCGCcaucauaauuagacGAGGGGUgGUGGGGCGa -3' miRNA: 3'- -GGGuUGCGa-------------CUCCCCGgUACUCUGUc -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 115065 | 0.7 | 0.676136 |
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Target: 5'- cCCCGGCGCccacaUGGGgcuGGGCCAUGuacggauacaAGACGGc -3' miRNA: 3'- -GGGUUGCG-----ACUC---CCCGGUAC----------UCUGUC- -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 146547 | 0.7 | 0.676136 |
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Target: 5'- cCCCGGCGCccacaUGGGgcuGGGCCAUGuacggauacaAGACGGc -3' miRNA: 3'- -GGGUUGCG-----ACUC---CCCGGUAC----------UCUGUC- -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 99589 | 0.7 | 0.655824 |
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Target: 5'- gCCAGCGUUGGcGGaGCCAUGGGAgGa -3' miRNA: 3'- gGGUUGCGACUcCC-CGGUACUCUgUc -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 115263 | 1.1 | 0.002322 |
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Target: 5'- uCCCAACGCUGAGGGGCCAUGAGACAGu -3' miRNA: 3'- -GGGUUGCGACUCCCCGGUACUCUGUC- -5' |
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| 31371 | 5' | -56.5 | NC_006623.1 | + | 146349 | 1.1 | 0.002322 |
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Target: 5'- uCCCAACGCUGAGGGGCCAUGAGACAGu -3' miRNA: 3'- -GGGUUGCGACUCCCCGGUACUCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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