Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31372 | 3' | -56.6 | NC_006623.1 | + | 52618 | 0.66 | 0.851767 |
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Target: 5'- -aGAGUCCAguCCCCcUUCCAccgcuuugaaacaGCCGUUa -3' miRNA: 3'- aaCUCGGGU--GGGGcAAGGU-------------UGGCAAg -5' |
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| 31372 | 3' | -56.6 | NC_006623.1 | + | 126898 | 0.74 | 0.409254 |
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Target: 5'- aUGAGUCCaaaccgACCCCGauaCAGCCGUUCa -3' miRNA: 3'- aACUCGGG------UGGGGCaagGUUGGCAAG- -5' |
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| 31372 | 3' | -56.6 | NC_006623.1 | + | 123070 | 0.76 | 0.335598 |
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Target: 5'- -cGGG-CgGCCCCGUUCC-ACCGUUCg -3' miRNA: 3'- aaCUCgGgUGGGGCAAGGuUGGCAAG- -5' |
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| 31372 | 3' | -56.6 | NC_006623.1 | + | 138542 | 0.76 | 0.335598 |
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Target: 5'- -cGGG-CgGCCCCGUUCC-ACCGUUCg -3' miRNA: 3'- aaCUCgGgUGGGGCAAGGuUGGCAAG- -5' |
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| 31372 | 3' | -56.6 | NC_006623.1 | + | 115321 | 1.08 | 0.002539 |
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Target: 5'- aUUGAGCCCACCCCGUUCCAACCGUUCg -3' miRNA: 3'- -AACUCGGGUGGGGCAAGGUUGGCAAG- -5' |
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| 31372 | 3' | -56.6 | NC_006623.1 | + | 146291 | 1.08 | 0.002539 |
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Target: 5'- aUUGAGCCCACCCCGUUCCAACCGUUCg -3' miRNA: 3'- -AACUCGGGUGGGGCAAGGUUGGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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