Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31379 | 5' | -57.7 | NC_006623.1 | + | 677 | 0.66 | 0.839138 |
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Target: 5'- uGCGGAGCCUCgCGaaauCCaa-CGGCGGGc- -3' miRNA: 3'- -UGCCUUGGAG-GU----GGaagGCCGCCUac -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 24044 | 0.66 | 0.820951 |
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Target: 5'- -gGGAGCaCUUCACCUcaaggacuauggUgGGCGGAUGa -3' miRNA: 3'- ugCCUUG-GAGGUGGAa-----------GgCCGCCUAC- -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 55358 | 0.67 | 0.814132 |
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Target: 5'- uCaGAACUUUCuCCUUCCGGgGGAUc -3' miRNA: 3'- uGcCUUGGAGGuGGAAGGCCgCCUAc -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 129867 | 0.67 | 0.796644 |
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Target: 5'- cGCGGGACgUCCGCCU-CagacGCGGAc- -3' miRNA: 3'- -UGCCUUGgAGGUGGAaGgc--CGCCUac -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 62645 | 0.68 | 0.74098 |
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Target: 5'- uAUGGAGacguCCgCCGCCUUCCuGCGGGg- -3' miRNA: 3'- -UGCCUU----GGaGGUGGAAGGcCGCCUac -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 110401 | 0.69 | 0.691966 |
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Target: 5'- -----cCCUCCACCauUUCCGGCGG-UGg -3' miRNA: 3'- ugccuuGGAGGUGG--AAGGCCGCCuAC- -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 123359 | 0.69 | 0.67196 |
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Target: 5'- -gGGAACCUUCGgggaggUCUUCCGGgaGGGUGa -3' miRNA: 3'- ugCCUUGGAGGU------GGAAGGCCg-CCUAC- -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 138253 | 0.69 | 0.67196 |
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Target: 5'- -gGGAACCUUCGgggaggUCUUCCGGgaGGGUGa -3' miRNA: 3'- ugCCUUGGAGGU------GGAAGGCCg-CCUAC- -5' |
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| 31379 | 5' | -57.7 | NC_006623.1 | + | 126193 | 1.07 | 0.002797 |
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Target: 5'- uACGGAACCUCCACCUUCCGGCGGAUGu -3' miRNA: 3'- -UGCCUUGGAGGUGGAAGGCCGCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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