Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31384 | 5' | -54.3 | NC_006623.1 | + | 38257 | 0.66 | 0.936681 |
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Target: 5'- gGAACgUGUGUUU---GCCCGGACg- -3' miRNA: 3'- gCUUGgACACGGAaagUGGGCCUGag -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 131314 | 0.68 | 0.896213 |
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Target: 5'- uGGGCagGUGCCUcgguuucugCACCCGGAgUCu -3' miRNA: 3'- gCUUGgaCACGGAaa-------GUGGGCCUgAG- -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 102357 | 0.68 | 0.867854 |
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Target: 5'- aGuAACgUGcUGCCagUUCAUCCGGugUCa -3' miRNA: 3'- gC-UUGgAC-ACGGa-AAGUGGGCCugAG- -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 123499 | 0.69 | 0.85235 |
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Target: 5'- gCGGACCgcgaGUGCgCcccUC-CCCGGGCUCu -3' miRNA: 3'- -GCUUGGa---CACG-Gaa-AGuGGGCCUGAG- -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 138113 | 0.69 | 0.85235 |
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Target: 5'- gCGGACCgcgaGUGCgCcccUC-CCCGGGCUCu -3' miRNA: 3'- -GCUUGGa---CACG-Gaa-AGuGGGCCUGAG- -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 131338 | 0.69 | 0.818941 |
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Target: 5'- uGAACCg--GCgaUUUUUACUCGGACUCa -3' miRNA: 3'- gCUUGGacaCG--GAAAGUGGGCCUGAG- -5' |
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| 31384 | 5' | -54.3 | NC_006623.1 | + | 131529 | 1.1 | 0.003556 |
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Target: 5'- cCGAACCUGUGCCUUUCACCCGGACUCu -3' miRNA: 3'- -GCUUGGACACGGAAAGUGGGCCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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