Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31387 | 5' | -59 | NC_006623.1 | + | 118914 | 0.66 | 0.797606 |
Target: 5'- gCGACUCUcgaaaccucCCGuuUCuCGCGUuGGCCUg -3' miRNA: 3'- -GCUGAGA---------GGCucAGuGCGCGuCCGGA- -5' |
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31387 | 5' | -59 | NC_006623.1 | + | 142698 | 0.66 | 0.797606 |
Target: 5'- gCGACUCUcgaaaccucCCGuuUCuCGCGUuGGCCUg -3' miRNA: 3'- -GCUGAGA---------GGCucAGuGCGCGuCCGGA- -5' |
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31387 | 5' | -59 | NC_006623.1 | + | 126941 | 0.67 | 0.751964 |
Target: 5'- gGACgggCCGAGUUggaACGCGCAGGa-- -3' miRNA: 3'- gCUGagaGGCUCAG---UGCGCGUCCgga -5' |
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31387 | 5' | -59 | NC_006623.1 | + | 144669 | 0.68 | 0.684064 |
Target: 5'- uGGCUgCUCCGAcgaggGUgACGUGC-GGCCUu -3' miRNA: 3'- gCUGA-GAGGCU-----CAgUGCGCGuCCGGA- -5' |
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31387 | 5' | -59 | NC_006623.1 | + | 116943 | 0.68 | 0.684064 |
Target: 5'- uGGCUgCUCCGAcgaggGUgACGUGC-GGCCUu -3' miRNA: 3'- gCUGA-GAGGCU-----CAgUGCGCGuCCGGA- -5' |
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31387 | 5' | -59 | NC_006623.1 | + | 135964 | 1.08 | 0.001862 |
Target: 5'- gCGACUCUCCGAGUCACGCGCAGGCCUg -3' miRNA: 3'- -GCUGAGAGGCUCAGUGCGCGUCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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