Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31388 | 5' | -57.3 | NC_006623.1 | + | 123705 | 0.67 | 0.782809 |
Target: 5'- gGUCcaCAGuccuaccgccGCUGCGAcGGCGCGCuuaGCCg -3' miRNA: 3'- -UAGaaGUC----------UGACGCUcCCGCGCG---UGG- -5' |
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31388 | 5' | -57.3 | NC_006623.1 | + | 137907 | 0.67 | 0.782809 |
Target: 5'- gGUCcaCAGuccuaccgccGCUGCGAcGGCGCGCuuaGCCg -3' miRNA: 3'- -UAGaaGUC----------UGACGCUcCCGCGCG---UGG- -5' |
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31388 | 5' | -57.3 | NC_006623.1 | + | 134937 | 0.69 | 0.664855 |
Target: 5'- uAUCggacaCGGACUGgGAGGG-GCGUACa -3' miRNA: 3'- -UAGaa---GUCUGACgCUCCCgCGCGUGg -5' |
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31388 | 5' | -57.3 | NC_006623.1 | + | 14205 | 0.71 | 0.58308 |
Target: 5'- aAUCgUCgAGGgUGCgGAGGGCGCGCAa- -3' miRNA: 3'- -UAGaAG-UCUgACG-CUCCCGCGCGUgg -5' |
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31388 | 5' | -57.3 | NC_006623.1 | + | 25727 | 0.75 | 0.369388 |
Target: 5'- uUCUgcaaCGGACUcuagaagcggugGCGGGGGCGCGCAaauCCa -3' miRNA: 3'- uAGAa---GUCUGA------------CGCUCCCGCGCGU---GG- -5' |
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31388 | 5' | -57.3 | NC_006623.1 | + | 136892 | 1.1 | 0.001571 |
Target: 5'- cAUCUUCAGACUGCGAGGGCGCGCACCa -3' miRNA: 3'- -UAGAAGUCUGACGCUCCCGCGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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