miRNA: 3'- ccACGGACaaCCGGUCuUAGC-------ACCUG- -5'
miRNA: 3'- -CCACGGACAA------CCGguCuua----GCACCUG- -5'
miRNA: 3'- ccaCGGAcaa-CCGG----UCUUAGCACCUG- -5'
miRNA: 3'- -CCACGGACAACCGGUCUUAGCACCUG- -5'
miRNA: 3'- -CCACGGACAACCGGUCUUAGCACCUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31396 | 3' | -54 | NC_006623.1 | + | 1778 | 0.67 | 0.935524 |
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Target: 5'- -aUGCCUGcgGGCUAG-GUCGcacuugcUGGGCa -3' miRNA: 3'- ccACGGACaaCCGGUCuUAGC-------ACCUG- -5' |
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| 31396 | 3' | -54 | NC_006623.1 | + | 28598 | 0.67 | 0.925562 |
|
Target: 5'- aGGUGCUUGUUgcauggGGCguGuacugggCGUGGAUa -3' miRNA: 3'- -CCACGGACAA------CCGguCuua----GCACCUG- -5' |
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| 31396 | 3' | -54 | NC_006623.1 | + | 54764 | 0.67 | 0.91411 |
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Target: 5'- --cGCCUuucaGGCCcucaAGGAUUGUGGACg -3' miRNA: 3'- ccaCGGAcaa-CCGG----UCUUAGCACCUG- -5' |
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| 31396 | 3' | -54 | NC_006623.1 | + | 145804 | 1.13 | 0.002241 |
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Target: 5'- cGGUGCCUGUUGGCCAGAAUCGUGGACa -3' miRNA: 3'- -CCACGGACAACCGGUCUUAGCACCUG- -5' |
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| 31396 | 3' | -54 | NC_006623.1 | + | 115807 | 1.13 | 0.002241 |
|
Target: 5'- cGGUGCCUGUUGGCCAGAAUCGUGGACa -3' miRNA: 3'- -CCACGGACAACCGGUCUUAGCACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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