miRNA: 3'- cauAGGGauaUAGGGAGGGCUGCUcu -5'
miRNA: 3'- cauAGGGauaUAGGGAGGGCUGCUcu -5'
miRNA: 3'- caUAGGGAUAuAGGGAG-GGCUGCuCU- -5'
miRNA: 3'- -CAUAGGGAUAUAGGGAgGGCUGCUCU- -5'
miRNA: 3'- -CAUAGGGAUAUAGGGAGGGCUGCUCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31396 | 5' | -52.6 | NC_006623.1 | + | 122556 | 0.68 | 0.936972 |
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Target: 5'- --cUCCCg--GUCUCUCCUGGCGGc- -3' miRNA: 3'- cauAGGGauaUAGGGAGGGCUGCUcu -5' |
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| 31396 | 5' | -52.6 | NC_006623.1 | + | 139056 | 0.68 | 0.936972 |
|
Target: 5'- --cUCCCg--GUCUCUCCUGGCGGc- -3' miRNA: 3'- cauAGGGauaUAGGGAGGGCUGCUcu -5' |
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| 31396 | 5' | -52.6 | NC_006623.1 | + | 713 | 0.69 | 0.915163 |
|
Target: 5'- -cAUUCCUAUcUCCCUCuCCGAUGcGGc -3' miRNA: 3'- caUAGGGAUAuAGGGAG-GGCUGCuCU- -5' |
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| 31396 | 5' | -52.6 | NC_006623.1 | + | 115772 | 0.96 | 0.041417 |
|
Target: 5'- aGUAUCCCUAUAUCCCU-CCGACGAGAa -3' miRNA: 3'- -CAUAGGGAUAUAGGGAgGGCUGCUCU- -5' |
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| 31396 | 5' | -52.6 | NC_006623.1 | + | 145839 | 1.1 | 0.005602 |
|
Target: 5'- aGUAUCCCUAUAUCCCUCCCGACGAGAa -3' miRNA: 3'- -CAUAGGGAUAUAGGGAGGGCUGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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