Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 314 | 3' | -60.6 | AC_000010.1 | + | 8557 | 0.66 | 0.303795 |
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Target: 5'- --cGGCgCCgacGUGCCacugccaGCCAuGCCGCCGg -3' miRNA: 3'- cuaCCGgGGa--UAUGG-------CGGU-CGGCGGU- -5' |
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| 314 | 3' | -60.6 | AC_000010.1 | + | 8518 | 0.67 | 0.268221 |
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Target: 5'- --cGaGCCgCCUcUACCGCCAGgCGCg- -3' miRNA: 3'- cuaC-CGG-GGAuAUGGCGGUCgGCGgu -5' |
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| 314 | 3' | -60.6 | AC_000010.1 | + | 4920 | 0.67 | 0.261388 |
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Target: 5'- aGUGGCCCCccUGCuccgagaggaCGgCAGCUGCCGc -3' miRNA: 3'- cUACCGGGGauAUG----------GCgGUCGGCGGU- -5' |
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| 314 | 3' | -60.6 | AC_000010.1 | + | 9349 | 0.67 | 0.241737 |
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Target: 5'- ---uGCCCgUGcGCCGCCGGCguCGCCGc -3' miRNA: 3'- cuacCGGGgAUaUGGCGGUCG--GCGGU- -5' |
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| 314 | 3' | -60.6 | AC_000010.1 | + | 14053 | 0.71 | 0.132679 |
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Target: 5'- uGGUGGCUgCaucGCCGCC-GCCGCCGc -3' miRNA: 3'- -CUACCGGgGauaUGGCGGuCGGCGGU- -5' |
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| 314 | 3' | -60.6 | AC_000010.1 | + | 10243 | 1.08 | 0.000174 |
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Target: 5'- cGAUGGCCCCUAUACCGCCAGCCGCCAc -3' miRNA: 3'- -CUACCGGGGAUAUGGCGGUCGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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