Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31409 | 3' | -50.1 | NC_006634.1 | + | 13329 | 0.66 | 0.565877 |
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Target: 5'- ---gAUCUGGCCauauauaaacAGAUCUgGcCAGAUc -3' miRNA: 3'- aauaUAGACCGG----------UCUAGAgCaGUCUA- -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 12990 | 0.68 | 0.45585 |
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Target: 5'- ---gAUCUGGCCAGAUauaaUCAGAUc -3' miRNA: 3'- aauaUAGACCGGUCUAgagcAGUCUA- -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 12954 | 0.68 | 0.444316 |
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Target: 5'- ---gAUCUGGCCAGAUCUuuaaaUGUCu--- -3' miRNA: 3'- aauaUAGACCGGUCUAGA-----GCAGucua -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 13626 | 0.68 | 0.444315 |
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Target: 5'- aUUAUAUCUGGCCAGAUauaacuauaUUUGUUAa-- -3' miRNA: 3'- -AAUAUAGACCGGUCUA---------GAGCAGUcua -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 13282 | 0.68 | 0.432947 |
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Target: 5'- ---gAUCUGGCCAGAUCU-GUuuauauauggcCAGAUc -3' miRNA: 3'- aauaUAGACCGGUCUAGAgCA-----------GUCUA- -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 12981 | 0.68 | 0.410727 |
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Target: 5'- ---aGUCUGGCCAGAUCU-GUuuauauauggcCAGAUc -3' miRNA: 3'- aauaUAGACCGGUCUAGAgCA-----------GUCUA- -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 13591 | 0.69 | 0.399887 |
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Target: 5'- gUUAUAUCUGGCCAGAuauaauaaaaUCUuuuuaUGUCuGAUu -3' miRNA: 3'- -AAUAUAGACCGGUCU----------AGA-----GCAGuCUA- -5' |
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| 31409 | 3' | -50.1 | NC_006634.1 | + | 13021 | 1.04 | 0.000924 |
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Target: 5'- aUUAUAUCUGGCCAGAUCUCGUCAGAUa -3' miRNA: 3'- -AAUAUAGACCGGUCUAGAGCAGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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