Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31409 | 5' | -45 | NC_006634.1 | + | 13017 | 0.7 | 0.658688 |
Target: 5'- -uAUCUGGCCAGAUCUcGUcagAUAg-- -3' miRNA: 3'- ucUAGACCGGUCUAGA-CAaa-UAUaua -5' |
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31409 | 5' | -45 | NC_006634.1 | + | 12988 | 0.72 | 0.539597 |
Target: 5'- gAGAUCUGGCCAGAuauaaucagaUCUGg-------- -3' miRNA: 3'- -UCUAGACCGGUCU----------AGACaaauauaua -5' |
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31409 | 5' | -45 | NC_006634.1 | + | 12952 | 0.8 | 0.208978 |
Target: 5'- cAGAUCUGGCCAGAUCU--UUAaAUGUc -3' miRNA: 3'- -UCUAGACCGGUCUAGAcaAAUaUAUA- -5' |
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31409 | 5' | -45 | NC_006634.1 | + | 13311 | 0.83 | 0.118882 |
Target: 5'- cAGAUCUGGCCAGAUCUGc-------- -3' miRNA: 3'- -UCUAGACCGGUCUAGACaaauauaua -5' |
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31409 | 5' | -45 | NC_006634.1 | + | 12981 | 0.99 | 0.010316 |
Target: 5'- -aGUCUGGCCAGAUCUGUUUAUAUAUg -3' miRNA: 3'- ucUAGACCGGUCUAGACAAAUAUAUA- -5' |
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31409 | 5' | -45 | NC_006634.1 | + | 13280 | 1.08 | 0.002342 |
Target: 5'- cAGAUCUGGCCAGAUCUGUUUAUAUAUg -3' miRNA: 3'- -UCUAGACCGGUCUAGACAAAUAUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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