Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31410 | 3' | -44.8 | NC_006634.1 | + | 13644 | 0.66 | 0.903311 |
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Target: 5'- cCCAUAUAUAGuuauaucuguuUAGAUCUGaUCAGAUa -3' miRNA: 3'- -GGUAUAUAUUu----------GUCUAGACcGGUCUA- -5' |
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| 31410 | 3' | -44.8 | NC_006634.1 | + | 13582 | 0.69 | 0.74383 |
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Target: 5'- aCAaAUAUAGuUAuAUCUGGCCAGAUa -3' miRNA: 3'- gGUaUAUAUUuGUcUAGACCGGUCUA- -5' |
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| 31410 | 3' | -44.8 | NC_006634.1 | + | 12996 | 0.73 | 0.509679 |
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Target: 5'- gCCAgAUAUAAuCAGAUCUGGUCAa-- -3' miRNA: 3'- -GGUaUAUAUUuGUCUAGACCGGUcua -5' |
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| 31410 | 3' | -44.8 | NC_006634.1 | + | 13260 | 0.81 | 0.177304 |
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Target: 5'- aCUAUAauUAUuagccuuAGCAGAUCUGGCCAGAUc -3' miRNA: 3'- -GGUAU--AUAu------UUGUCUAGACCGGUCUA- -5' |
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| 31410 | 3' | -44.8 | NC_006634.1 | + | 12940 | 1.09 | 0.002419 |
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Target: 5'- gCCAUAUAUAAACAGAUCUGGCCAGAUc -3' miRNA: 3'- -GGUAUAUAUUUGUCUAGACCGGUCUA- -5' |
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| 31410 | 3' | -44.8 | NC_006634.1 | + | 13323 | 1.09 | 0.002419 |
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Target: 5'- gCCAUAUAUAAACAGAUCUGGCCAGAUc -3' miRNA: 3'- -GGUAUAUAUUUGUCUAGACCGGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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