Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31413 | 5' | -51.7 | NC_006635.1 | + | 18811 | 0.69 | 0.588795 |
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Target: 5'- --------gUGCCACUUUCCGGCgGa -3' miRNA: 3'- ggucuuucaACGGUGAAAGGCCGgCc -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6606 | 0.73 | 0.348418 |
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Target: 5'- ---cAAAGUUGCCACaUUCCGGCUa- -3' miRNA: 3'- ggucUUUCAACGGUGaAAGGCCGGcc -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6197 | 0.73 | 0.348418 |
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Target: 5'- ---cAAAGUUGCCACgUUCCGGCUa- -3' miRNA: 3'- ggucUUUCAACGGUGaAAGGCCGGcc -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6233 | 0.77 | 0.214922 |
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Target: 5'- gCCGGAAAGUgGaaACUUUCUGGCCGc -3' miRNA: 3'- -GGUCUUUCAaCggUGAAAGGCCGGCc -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6570 | 0.8 | 0.127757 |
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Target: 5'- gCCGGAAAGUgGCaACUUUCUGGCCGc -3' miRNA: 3'- -GGUCUUUCAaCGgUGAAAGGCCGGCc -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6181 | 1.01 | 0.004721 |
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Target: 5'- gCCAGAAAGUUuCCACUUUCCGGCCGGa -3' miRNA: 3'- -GGUCUUUCAAcGGUGAAAGGCCGGCC- -5' |
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| 31413 | 5' | -51.7 | NC_006635.1 | + | 6622 | 1.14 | 0.000583 |
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Target: 5'- gCCAGAAAGUUGCCACUUUCCGGCCGGa -3' miRNA: 3'- -GGUCUUUCAACGGUGAAAGGCCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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