Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31415 | 3' | -41.4 | NC_006635.1 | + | 25565 | 0.68 | 0.995794 |
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Target: 5'- ----aGCCCgcUUGGUgugUGGAAGACa -3' miRNA: 3'- auaugCGGGaaAAUCGaa-AUCUUUUG- -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 24785 | 0.81 | 0.553872 |
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Target: 5'- uUAUACGCCCUUUUGGCa-UGGAAc-- -3' miRNA: 3'- -AUAUGCGGGAAAAUCGaaAUCUUuug -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 24745 | 0.83 | 0.432374 |
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Target: 5'- aAUACGCCCUUUUGGCaUUAGGc--- -3' miRNA: 3'- aUAUGCGGGAAAAUCGaAAUCUuuug -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26722 | 0.84 | 0.409948 |
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Target: 5'- -cUACGgCCUUUUGGCUUUGGAAAu- -3' miRNA: 3'- auAUGCgGGAAAAUCGAAAUCUUUug -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26806 | 0.85 | 0.377701 |
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Target: 5'- uUAUACGCCCUcUUGGCUUUGcAAAGCc -3' miRNA: 3'- -AUAUGCGGGAaAAUCGAAAUcUUUUG- -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26852 | 0.85 | 0.377701 |
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Target: 5'- uUAUACGCCCUUUUGGCaUUAGGcacGCg -3' miRNA: 3'- -AUAUGCGGGAAAAUCGaAAUCUuu-UG- -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26873 | 0.87 | 0.274725 |
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Target: 5'- --cACGCCCUUUUGGCUUUgcaaagccaAGAGGGCg -3' miRNA: 3'- auaUGCGGGAAAAUCGAAA---------UCUUUUG- -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26650 | 1 | 0.049595 |
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Target: 5'- uUAUACGCCCUUUUGGCUUUAGAucACu -3' miRNA: 3'- -AUAUGCGGGAAAAUCGAAAUCUuuUG- -5' |
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| 31415 | 3' | -41.4 | NC_006635.1 | + | 26742 | 1.1 | 0.010639 |
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Target: 5'- uUAUACGCCCUUUUAGCUUUAGAAAACu -3' miRNA: 3'- -AUAUGCGGGAAAAUCGAAAUCUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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