Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31415 | 5' | -48.2 | NC_006635.1 | + | 9554 | 0.66 | 0.878169 |
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Target: 5'- uUAGUaucGAUCgucuAGUC-AGAGGGCGUAc -3' miRNA: 3'- -AUCA---CUAGauu-UCGGuUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 1570 | 0.69 | 0.69424 |
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Target: 5'- cGG-GAUUaAAAGUCAAGAGGGUGUc -3' miRNA: 3'- aUCaCUAGaUUUCGGUUUUCCCGCAu -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 26818 | 0.73 | 0.498391 |
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Target: 5'- --uUGG-CUuugcAAAGCCAAAAGGGCGUGu -3' miRNA: 3'- aucACUaGA----UUUCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 24728 | 0.73 | 0.498391 |
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Target: 5'- aUAG-GGUUccauGCCAAAAGGGCGUAu -3' miRNA: 3'- -AUCaCUAGauuuCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 26864 | 0.73 | 0.452655 |
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Target: 5'- --uUGG-CUuugcAAAGCCAAGAGGGCGUAu -3' miRNA: 3'- aucACUaGA----UUUCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 24691 | 0.76 | 0.340005 |
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Target: 5'- -cGUGc-CUAAuGCCAAAAGGGCGUAu -3' miRNA: 3'- auCACuaGAUUuCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 26906 | 0.76 | 0.340005 |
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Target: 5'- -cGUGc-CUAAuGCCAAAAGGGCGUAu -3' miRNA: 3'- auCACuaGAUUuCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 26685 | 0.85 | 0.084015 |
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Target: 5'- gAGUuuUCUAAAGCUAAAAGGGCGUAu -3' miRNA: 3'- aUCAcuAGAUUUCGGUUUUCCCGCAU- -5' |
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| 31415 | 5' | -48.2 | NC_006635.1 | + | 26708 | 1.06 | 0.003085 |
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Target: 5'- uUAGUGAUCUAAAGCCAAAAGGGCGUAu -3' miRNA: 3'- -AUCACUAGAUUUCGGUUUUCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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