Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31416 | 3' | -53 | NC_006635.1 | + | 26810 | 0.68 | 0.518095 |
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Target: 5'- aCGCccucuugGCUUugcaaaGCCAAAAGGGCGUGu -3' miRNA: 3'- -GCGca-----CGGAuua---CGGUUUUCCCGCAU- -5' |
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| 31416 | 3' | -53 | NC_006635.1 | + | 26872 | 0.68 | 0.473529 |
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Target: 5'- aCGCccuuuugGCUUugcaaaGCCAAGAGGGCGUAu -3' miRNA: 3'- -GCGca-----CGGAuua---CGGUUUUCCCGCAU- -5' |
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| 31416 | 3' | -53 | NC_006635.1 | + | 24730 | 0.73 | 0.255907 |
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Target: 5'- aGgGUuCC--AUGCCAAAAGGGCGUAu -3' miRNA: 3'- gCgCAcGGauUACGGUUUUCCCGCAU- -5' |
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| 31416 | 3' | -53 | NC_006635.1 | + | 26706 | 0.74 | 0.204115 |
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Target: 5'- --aGUGaUCUAAaGCCAAAAGGGCGUAu -3' miRNA: 3'- gcgCAC-GGAUUaCGGUUUUCCCGCAU- -5' |
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| 31416 | 3' | -53 | NC_006635.1 | + | 24688 | 1.07 | 0.000884 |
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Target: 5'- uCGCGUGCCUAAUGCCAAAAGGGCGUAu -3' miRNA: 3'- -GCGCACGGAUUACGGUUUUCCCGCAU- -5' |
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| 31416 | 3' | -53 | NC_006635.1 | + | 26909 | 1.07 | 0.000884 |
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Target: 5'- uCGCGUGCCUAAUGCCAAAAGGGCGUAu -3' miRNA: 3'- -GCGCACGGAUUACGGUUUUCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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