miRNA: 3'- -UCGG-AGuCUAGUgUACAAa--CAGUugG- -5'
miRNA: 3'- -UCGGAGUCUaGUGUACA--AACaGUUGG- -5'
miRNA: 3'- -UCGGAGUCUAGUGUACA--AACAGUUGG- -5'
miRNA: 3'- -UCGGAGUCUaGUGUACAAACAGUUGG- -5'
miRNA: 3'- -UCGGAGUCUAGUGUACAAACAGUUGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31420 | 3' | -47 | NC_006635.1 | + | 12569 | 0.68 | 0.885446 |
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Target: 5'- aAGCCuUCuGAUCAaGUGUUggaGUCAugCu -3' miRNA: 3'- -UCGG-AGuCUAGUgUACAAa--CAGUugG- -5' |
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| 31420 | 3' | -47 | NC_006635.1 | + | 6033 | 0.74 | 0.561545 |
|
Target: 5'- aAGUCUCAGAcCACAUGUaaUUGcUGGCCg -3' miRNA: 3'- -UCGGAGUCUaGUGUACA--AACaGUUGG- -5' |
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| 31420 | 3' | -47 | NC_006635.1 | + | 6770 | 0.94 | 0.03773 |
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Target: 5'- aAGUCUCAGAUCACAUGUaaUUGUUGGCCg -3' miRNA: 3'- -UCGGAGUCUAGUGUACA--AACAGUUGG- -5' |
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| 31420 | 3' | -47 | NC_006635.1 | + | 6130 | 1.04 | 0.007488 |
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Target: 5'- aAGCCUCAGAcCACAUGUUUGUCAACCu -3' miRNA: 3'- -UCGGAGUCUaGUGUACAAACAGUUGG- -5' |
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| 31420 | 3' | -47 | NC_006635.1 | + | 6673 | 1.14 | 0.001622 |
|
Target: 5'- aAGCCUCAGAUCACAUGUUUGUCAACCu -3' miRNA: 3'- -UCGGAGUCUAGUGUACAAACAGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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