Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31421 | 3' | -46.3 | NC_006635.1 | + | 4902 | 0.66 | 0.94996 |
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Target: 5'- -uUACGCUUgUUUGGCgUUAGAUUuuACa -3' miRNA: 3'- auAUGCGGGaAAACCGaAAUCUAG--UG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26806 | 0.73 | 0.596742 |
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Target: 5'- uUAUACGCCCUcUUGGCUUUGcaaaGCc -3' miRNA: 3'- -AUAUGCGGGAaAACCGAAAUcuagUG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 24785 | 0.76 | 0.441796 |
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Target: 5'- uUAUACGCCCUUUUGGCa-UGGAa--- -3' miRNA: 3'- -AUAUGCGGGAAAACCGaaAUCUagug -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26722 | 0.77 | 0.419927 |
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Target: 5'- -cUACGgCCUUUUGGCUUUGGAa--- -3' miRNA: 3'- auAUGCgGGAAAACCGAAAUCUagug -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26873 | 0.78 | 0.368242 |
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Target: 5'- --cACGCCCUUUUGGCUUUgcaaagccaAGAggGCg -3' miRNA: 3'- auaUGCGGGAAAACCGAAA---------UCUagUG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26742 | 0.8 | 0.278612 |
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Target: 5'- uUAUACGCCCUUUUaGCUUUAGAaaACu -3' miRNA: 3'- -AUAUGCGGGAAAAcCGAAAUCUagUG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 24745 | 0.88 | 0.085523 |
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Target: 5'- aAUACGCCCUUUUGGCaUUAGG-CACg -3' miRNA: 3'- aUAUGCGGGAAAACCGaAAUCUaGUG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26852 | 0.89 | 0.072619 |
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Target: 5'- uUAUACGCCCUUUUGGCaUUAGG-CACg -3' miRNA: 3'- -AUAUGCGGGAAAACCGaAAUCUaGUG- -5' |
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| 31421 | 3' | -46.3 | NC_006635.1 | + | 26650 | 1.09 | 0.00316 |
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Target: 5'- uUAUACGCCCUUUUGGCUUUAGAUCACu -3' miRNA: 3'- -AUAUGCGGGAAAACCGAAAUCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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