miRNA: 3'- cuCAaaaGAUUuCGaUUUUCCCGCAU- -5'
miRNA: 3'- cuCAaaaGAUUuCGaUUUUCCCGCAU- -5'
miRNA: 3'- cUCAaaAGAUUUCGaUUUUCCCGCAU- -5'
miRNA: 3'- -CUCAAAAGAUUUCGAUUUUCCCGCAU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31421 | 5' | -43.5 | NC_006635.1 | + | 24691 | 0.66 | 0.992393 |
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Target: 5'- -cGUgc-CUAAuGCcAAAAGGGCGUAu -3' miRNA: 3'- cuCAaaaGAUUuCGaUUUUCCCGCAU- -5' |
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| 31421 | 5' | -43.5 | NC_006635.1 | + | 26906 | 0.66 | 0.992393 |
|
Target: 5'- -cGUgc-CUAAuGCcAAAAGGGCGUAu -3' miRNA: 3'- cuCAaaaGAUUuCGaUUUUCCCGCAU- -5' |
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| 31421 | 5' | -43.5 | NC_006635.1 | + | 26707 | 0.83 | 0.318338 |
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Target: 5'- uAGUgaUCUAAAGCcAAAAGGGCGUAu -3' miRNA: 3'- cUCAaaAGAUUUCGaUUUUCCCGCAU- -5' |
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| 31421 | 5' | -43.5 | NC_006635.1 | + | 26684 | 1.08 | 0.008257 |
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Target: 5'- uGAGUUUUCUAAAGCUAAAAGGGCGUAu -3' miRNA: 3'- -CUCAAAAGAUUUCGAUUUUCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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