Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31422 | 5' | -44.4 | NC_006635.1 | + | 24700 | 0.69 | 0.929146 |
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Target: 5'- uGCCAAAAGGGCGUauuACa------ -3' miRNA: 3'- uCGGUUUUCCCGCAcauUGaaaaaua -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 24739 | 0.69 | 0.922476 |
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Target: 5'- uGCCAAAAGGGCGUaUAAa------- -3' miRNA: 3'- uCGGUUUUCCCGCAcAUUgaaaaaua -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 26897 | 0.7 | 0.900263 |
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Target: 5'- uGCCAAAAGGGCGUaUAAaag-UUAc -3' miRNA: 3'- uCGGUUUUCCCGCAcAUUgaaaAAUa -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 26695 | 0.78 | 0.461838 |
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Target: 5'- aAGCUAAAAGGGCGUaUAACUUg---- -3' miRNA: 3'- -UCGGUUUUCCCGCAcAUUGAAaaaua -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 26697 | 0.82 | 0.282889 |
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Target: 5'- aAGCCAAAAGGGCGUaUAAUUUUUa-- -3' miRNA: 3'- -UCGGUUUUCCCGCAcAUUGAAAAaua -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 26853 | 0.83 | 0.259005 |
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Target: 5'- aAGCCAAGAGGGCGUaUAAUUUUUUu- -3' miRNA: 3'- -UCGGUUUUCCCGCAcAUUGAAAAAua -5' |
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| 31422 | 5' | -44.4 | NC_006635.1 | + | 26829 | 1.08 | 0.006662 |
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Target: 5'- aAGCCAAAAGGGCGUGUAACUUUUUAUa -3' miRNA: 3'- -UCGGUUUUCCCGCACAUUGAAAAAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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