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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31427 | 3' | -41.2 | NC_006639.1 | + | 12118 | 0.66 | 0.99827 |
Target: 5'- -aUUUGUgACUgGCUGCAGaACAAAGg -3' miRNA: 3'- uaAAAUAgUGAaUGGUGUC-UGUUUCg -5' |
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31427 | 3' | -41.2 | NC_006639.1 | + | 12335 | 0.75 | 0.817069 |
Target: 5'- uGUUUUAUCACUUuaCACAGGCcaAGAGUg -3' miRNA: 3'- -UAAAAUAGUGAAugGUGUCUG--UUUCG- -5' |
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31427 | 3' | -41.2 | NC_006639.1 | + | 12425 | 1.14 | 0.006033 |
Target: 5'- uAUUUUAUCACUUACCACAGACAAAGCa -3' miRNA: 3'- -UAAAAUAGUGAAUGGUGUCUGUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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