Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31442 | 3' | -39 | NC_006645.1 | + | 4080 | 0.66 | 0.999987 |
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Target: 5'- aCUGUACUGacc-AGGuuuucuGUGGUUUUCc -3' miRNA: 3'- gGAUAUGAUaauaUCCu-----UACCAAAGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5393 | 0.67 | 0.999845 |
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Target: 5'- uCCUGUAgcAUUAUGGGAaccauucccauaAUGGUauaggaauagUUCCc -3' miRNA: 3'- -GGAUAUgaUAAUAUCCU------------UACCA----------AAGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5408 | 0.68 | 0.999776 |
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Target: 5'- uCCUAUACUAUUAUAGaaacua-UUCCu -3' miRNA: 3'- -GGAUAUGAUAAUAUCcuuaccaAAGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5209 | 0.72 | 0.99151 |
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Target: 5'- uCCUAUAauagUAUAGGAAUGa-UUCCu -3' miRNA: 3'- -GGAUAUgauaAUAUCCUUACcaAAGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5202 | 0.74 | 0.96838 |
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Target: 5'- uCCcAUAauagUAUAGGAAUGGUUcCCa -3' miRNA: 3'- -GGaUAUgauaAUAUCCUUACCAAaGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5362 | 0.81 | 0.718057 |
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Target: 5'- uCCcAUAaUAUUAUGGGAAUGGUUcCCa -3' miRNA: 3'- -GGaUAUgAUAAUAUCCUUACCAAaGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5293 | 0.93 | 0.223003 |
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Target: 5'- uCCUAUAaUAUUAUGGGAAUGGUUcCCa -3' miRNA: 3'- -GGAUAUgAUAAUAUCCUUACCAAaGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5453 | 0.97 | 0.137038 |
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Target: 5'- uCCUAUACcAUUAUGGGAAUGGUUcCCa -3' miRNA: 3'- -GGAUAUGaUAAUAUCCUUACCAAaGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5164 | 1.08 | 0.028946 |
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Target: 5'- uCCUAUACUAUUAUGGGAAUGGUUcCCa -3' miRNA: 3'- -GGAUAUGAUAAUAUCCUUACCAAaGG- -5' |
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| 31442 | 3' | -39 | NC_006645.1 | + | 5247 | 1.2 | 0.005434 |
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Target: 5'- uCCUAUACUAUUAUAGGAAUGGUUUCCa -3' miRNA: 3'- -GGAUAUGAUAAUAUCCUUACCAAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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