Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31443 | 3' | -43.6 | NC_006645.1 | + | 5301 | 0.66 | 0.987794 |
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Target: 5'- uCCUAUAGcaUUAUGGGAAcCAUUCCc -3' miRNA: 3'- -GGGUAUUacGAUAUCCUUaGUAAGGa -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5372 | 0.69 | 0.911842 |
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Target: 5'- -nUAUAAUagUAUAGGAAUUAUUCCUg -3' miRNA: 3'- ggGUAUUAcgAUAUCCUUAGUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5430 | 0.75 | 0.649212 |
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Target: 5'- uCCCAUAAUGUac-AGGAAUaAUUCCUa -3' miRNA: 3'- -GGGUAUUACGauaUCCUUAgUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5209 | 0.76 | 0.597713 |
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Target: 5'- uCCUAUAAUagUAUAGGAAUgAUUCCUa -3' miRNA: 3'- -GGGUAUUAcgAUAUCCUUAgUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5347 | 0.77 | 0.521908 |
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Target: 5'- uCCCAUAAUGaUAUAGGAAUaGUUUCUa -3' miRNA: 3'- -GGGUAUUACgAUAUCCUUAgUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5202 | 0.78 | 0.497427 |
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Target: 5'- uCCCAUAAUagUAUAGGAAUgGUUCCc -3' miRNA: 3'- -GGGUAUUAcgAUAUCCUUAgUAAGGa -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5255 | 0.78 | 0.461717 |
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Target: 5'- uCCCAUAAUaUUAUAGGAAUaAUUCCUg -3' miRNA: 3'- -GGGUAUUAcGAUAUCCUUAgUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5416 | 0.85 | 0.213771 |
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Target: 5'- uCCCAUAAUGgUAUAGGAAUaGUUCCc -3' miRNA: 3'- -GGGUAUUACgAUAUCCUUAgUAAGGa -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5339 | 1.13 | 0.003343 |
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Target: 5'- uCCCAUAAUGCUAUAGGAAUCAUUCCUa -3' miRNA: 3'- -GGGUAUUACGAUAUCCUUAGUAAGGA- -5' |
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| 31443 | 3' | -43.6 | NC_006645.1 | + | 5270 | 1.13 | 0.003343 |
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Target: 5'- uCCCAUAAUGCUAUAGGAAUCAUUCCUa -3' miRNA: 3'- -GGGUAUUACGAUAUCCUUAGUAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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