Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31444 | 3' | -43.2 | NC_006645.1 | + | 5164 | 0.66 | 0.986774 |
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Target: 5'- uCCUAUAcuaUUAUGGGAAUGGUUCCc -3' miRNA: 3'- -GGGUAUuacGAUGUCCUUAUUAAGGa -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 518 | 0.68 | 0.957262 |
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Target: 5'- gCCAUGAUGCccgcagUGCAGGAAUcucaacuUUCUg -3' miRNA: 3'- gGGUAUUACG------AUGUCCUUAuu-----AAGGa -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5209 | 0.81 | 0.366347 |
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Target: 5'- uCCUAUAAUagUAUAGGAAUGAUUCCUa -3' miRNA: 3'- -GGGUAUUAcgAUGUCCUUAUUAAGGA- -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5202 | 0.81 | 0.366347 |
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Target: 5'- uCCCAUAAUagUAUAGGAAUGGUUCCc -3' miRNA: 3'- -GGGUAUUAcgAUGUCCUUAUUAAGGa -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5347 | 0.86 | 0.183974 |
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Target: 5'- uCCCAUAAUGaUAUAGGAAUAGUUUCUa -3' miRNA: 3'- -GGGUAUUACgAUGUCCUUAUUAAGGA- -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5255 | 0.88 | 0.142282 |
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Target: 5'- uCCCAUAAUaUUAUAGGAAUAAUUCCUg -3' miRNA: 3'- -GGGUAUUAcGAUGUCCUUAUUAAGGA- -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5416 | 0.94 | 0.063897 |
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Target: 5'- uCCCAUAAUGgUAUAGGAAUAGUUCCc -3' miRNA: 3'- -GGGUAUUACgAUGUCCUUAUUAAGGa -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5430 | 0.96 | 0.043867 |
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Target: 5'- uCCCAUAAUG-UACAGGAAUAAUUCCUa -3' miRNA: 3'- -GGGUAUUACgAUGUCCUUAUUAAGGA- -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5270 | 0.99 | 0.028007 |
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Target: 5'- uCCCAUAAUGCUAUAGGAAUcAUUCCUa -3' miRNA: 3'- -GGGUAUUACGAUGUCCUUAuUAAGGA- -5' |
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| 31444 | 3' | -43.2 | NC_006645.1 | + | 5339 | 0.99 | 0.028007 |
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Target: 5'- uCCCAUAAUGCUAUAGGAAUcAUUCCUa -3' miRNA: 3'- -GGGUAUUACGAUGUCCUUAuUAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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