Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3145 | 3' | -54 | NC_001499.1 | + | 163 | 1.12 | 0.000025 |
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Target: 5'- gGACGAGCCCCCAAAUGAAAGACCCCCg -3' miRNA: 3'- -CUGCUCGGGGGUUUACUUUCUGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 1220 | 0.73 | 0.05147 |
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Target: 5'- aGACggGAGCCCCC-AGUGGccGACUCCa -3' miRNA: 3'- -CUG--CUCGGGGGuUUACUuuCUGGGGg -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 4597 | 0.68 | 0.125548 |
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Target: 5'- ---uAGUCUCCAgaaaaagggggAAUGAAAGACCCCa -3' miRNA: 3'- cugcUCGGGGGU-----------UUACUUUCUGGGGg -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 3070 | 0.67 | 0.138762 |
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Target: 5'- cGACGgcuaAGCCUCCAG-----GGACUCCCa -3' miRNA: 3'- -CUGC----UCGGGGGUUuacuuUCUGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 394 | 0.67 | 0.158365 |
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Target: 5'- aACG-GCCCCCGAAgucccuUGGAcGuCUCCCa -3' miRNA: 3'- cUGCuCGGGGGUUU------ACUUuCuGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 4878 | 0.67 | 0.163642 |
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Target: 5'- aGAUG-GUCCCCAGAUGcgguccaGCCCUCa -3' miRNA: 3'- -CUGCuCGGGGGUUUACuuuc---UGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 5702 | 0.67 | 0.163642 |
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Target: 5'- aGAUG-GUCCCCAGAUGcgguccaGCCCUCa -3' miRNA: 3'- -CUGCuCGGGGGUUUACuuuc---UGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 5431 | 0.66 | 0.180435 |
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Target: 5'- cACGA--UUCUggGUGAAAGACCCCa -3' miRNA: 3'- cUGCUcgGGGGuuUACUUUCUGGGGg -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 148 | 0.66 | 0.186363 |
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Target: 5'- --gGGGCUcguCCgGGAUcGGGAGACCCCUg -3' miRNA: 3'- cugCUCGG---GGgUUUA-CUUUCUGGGGG- -5' |
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| 3145 | 3' | -54 | NC_001499.1 | + | 5227 | 0.66 | 0.192462 |
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Target: 5'- aGAaacuGUUaCCCA--UGAAAGACCCCCg -3' miRNA: 3'- -CUgcu-CGG-GGGUuuACUUUCUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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