Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31454 | 3' | -45.3 | NC_006646.1 | + | 1165 | 0.68 | 0.942144 |
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Target: 5'- aCAGGCUUGaUCAgGUCUGaUCAgGUCu -3' miRNA: 3'- -GUUCGGAUaAGUaCAGACaAGUaCAG- -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1337 | 0.76 | 0.580762 |
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Target: 5'- uCAAGCCUGUUCAUGcCUGcUCAUu-- -3' miRNA: 3'- -GUUCGGAUAAGUACaGACaAGUAcag -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1377 | 0.77 | 0.496197 |
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Target: 5'- aCAGGCUUGaUCAgGUCUGUUCAgGUCu -3' miRNA: 3'- -GUUCGGAUaAGUaCAGACAAGUaCAG- -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1061 | 0.87 | 0.138403 |
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Target: 5'- cCAuGUCUGUUCAUGcCUGUUCAUGUCu -3' miRNA: 3'- -GUuCGGAUAAGUACaGACAAGUACAG- -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1209 | 0.92 | 0.07046 |
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Target: 5'- uCAAGCCUGUcCAUGcCUGUUCAUGUCu -3' miRNA: 3'- -GUUCGGAUAaGUACaGACAAGUACAG- -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1125 | 1.01 | 0.01629 |
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Target: 5'- uCAAGCCUGUUCAUGUCUGcUCAUGUCu -3' miRNA: 3'- -GUUCGGAUAAGUACAGACaAGUACAG- -5' |
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| 31454 | 3' | -45.3 | NC_006646.1 | + | 1273 | 1.11 | 0.003943 |
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Target: 5'- uCAAGCCUAUUCAUGUCUGUUCAUGUCu -3' miRNA: 3'- -GUUCGGAUAAGUACAGACAAGUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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