Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31454 | 5' | -47.5 | NC_006646.1 | + | 1127 | 0.68 | 0.866217 |
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Target: 5'- ----aACgGUUCAGACaUGAACAGGCa -3' miRNA: 3'- auuuaUG-CGAGUCUGgACUUGUCUGg -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1275 | 0.69 | 0.80775 |
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Target: 5'- ----aAUGCuUCAGACaUGAACAGGCa -3' miRNA: 3'- auuuaUGCG-AGUCUGgACUUGUCUGg -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1317 | 0.69 | 0.797138 |
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Target: 5'- aUGAAUAgGCuugaUCAGGUCUGAuCAGGCCu -3' miRNA: 3'- -AUUUAUgCG----AGUCUGGACUuGUCUGG- -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1204 | 0.7 | 0.764094 |
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Target: 5'- cAGAUAUGaaCAGGCaUGAACAGACa -3' miRNA: 3'- aUUUAUGCgaGUCUGgACUUGUCUGg -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1020 | 0.77 | 0.393698 |
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Target: 5'- cAGGUAUGaUCAGGCCUGAAauUAGGCCu -3' miRNA: 3'- aUUUAUGCgAGUCUGGACUU--GUCUGG- -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1180 | 0.96 | 0.022718 |
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Target: 5'- cAAAUACGCUCAGACCUGAuCAGAUCu -3' miRNA: 3'- aUUUAUGCGAGUCUGGACUuGUCUGG- -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1243 | 1.02 | 0.009989 |
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Target: 5'- uUAAAUACGCUCAGGCCUGAuCAGACCu -3' miRNA: 3'- -AUUUAUGCGAGUCUGGACUuGUCUGG- -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1095 | 1.03 | 0.00793 |
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Target: 5'- uUAGAUACGCUCAGACCUGAuCAGACCu -3' miRNA: 3'- -AUUUAUGCGAGUCUGGACUuGUCUGG- -5' |
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| 31454 | 5' | -47.5 | NC_006646.1 | + | 1307 | 1.12 | 0.002045 |
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Target: 5'- uUAAAUACGCUCAGACCUGAACAGACCu -3' miRNA: 3'- -AUUUAUGCGAGUCUGGACUUGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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