Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31458 | 3' | -42.4 | NC_006649.1 | + | 5565 | 1.01 | 0.050059 |
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Target: 5'- uCCCAUAAaaUUAUGGGAAUAGUUCCCa -3' miRNA: 3'- -GGGUAUUaaAAUACCCUUGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5522 | 0.96 | 0.090142 |
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Target: 5'- uCCCAUAAUaUUAUGGGAuUAGUUCCCa -3' miRNA: 3'- -GGGUAUUAaAAUACCCUuGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5520 | 0.82 | 0.525374 |
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Target: 5'- uCCUAUAAUaUUAUaGGAAUGGUUCCCa -3' miRNA: 3'- -GGGUAUUAaAAUAcCCUUGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5474 | 0.87 | 0.297996 |
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Target: 5'- uCCCAUAAUaUUAUGGGAAUGGUaCCUa -3' miRNA: 3'- -GGGUAUUAaAAUACCCUUGUCAaGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5430 | 0.91 | 0.191773 |
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Target: 5'- uCCCAUAAUggUAUaGGAAUAGUUCCCa -3' miRNA: 3'- -GGGUAUUAaaAUAcCCUUGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5382 | 0.89 | 0.250813 |
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Target: 5'- uCCUAUAccaUUAUGGGAAUGGUUCCCa -3' miRNA: 3'- -GGGUAUuaaAAUACCCUUGUCAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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