Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31458 | 3' | -42.4 | NC_006649.1 | + | 31597 | 0.86 | 0.351776 |
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Target: 5'- uCCUAgaa--UUAUGGGAACAGUUCCUa -3' miRNA: 3'- -GGGUauuaaAAUACCCUUGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 31392 | 0.78 | 0.722257 |
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Target: 5'- uUCCAUAAUaUUAUGGaauUAGUUCCCa -3' miRNA: 3'- -GGGUAUUAaAAUACCcuuGUCAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 31461 | 0.73 | 0.926348 |
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Target: 5'- uCUUAUAGcaUUAUGGGAACcaUUCCCa -3' miRNA: 3'- -GGGUAUUaaAAUACCCUUGucAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 24088 | 0.69 | 0.992892 |
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Target: 5'- gCCAaGAUUaug-GGGGGCAGUgaaauucauugcaUCCCg -3' miRNA: 3'- gGGUaUUAAaauaCCCUUGUCA-------------AGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 5568 | 0.66 | 0.999551 |
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Target: 5'- uCCUAUAAUaUUAUaGGAAUAaUUCCUa -3' miRNA: 3'- -GGGUAUUAaAAUAcCCUUGUcAAGGG- -5' |
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| 31458 | 3' | -42.4 | NC_006649.1 | + | 31686 | 1.19 | 0.00391 |
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Target: 5'- uCCCAUAAUUUUAUGGGAACAGUUCCCa -3' miRNA: 3'- -GGGUAUUAAAAUACCCUUGUCAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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